來源于Escherichia coli（E.coli）的片段DNA可以用作核酸酶靶向切割和釋放（CUT&RUN）的實驗標準化的spike-in對照。產品CUTANA™ E. coli Spike-in DNA含有足夠的材料，可用于100-200個CUT&RUN樣本（高豐度和低豐度靶標的范圍）。
產品詳情
保存溫度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.
運輸溫度: Room temp. Can go on frozen cold packs or dry ice.
產品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.
驗證數據
 | Figure 1: DNA gel data E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E. coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions of DNA molecular weight markers are indicated. |
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Figure 2: CUT&RUN sequencing data CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher 13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE: Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 - 5%) of total sequencing reads. |
訂購詳情
貨號 | 產品名稱 | 規格 |
18-1401 | CUTANA™ E. coli Spike-in DNA | 100 ng |
如需了解更多詳細信息或相關產品，請聯系EpiCypher中國授權代理商-欣博盛生物
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